![]() They evaluated the DNA yield, and potential amplification of the extracted DNA by PCR using their own cells genetic material. ![]() The students were challenged to use three different protocols for DNA isolation (one using commercial silica column and two, in-house) using no lab animals but themselves. The course is five days long and emphasizes basic procedures in practical activities. In this work, we report a practical course designed for undergraduate students of health, biological sciences and biotechnology areas. Molecular Biology, Practical Course, DNA Extraction, Whole Blood, Polymerase Chain ReactionĪBSTRACT: DNA extraction and polymerase chain reaction are molecular biology techniques widely used in research, diagnostics and biotechnology industry. The Use of DNA Extraction for Molecular Biology and Biotechnology Training: A Practical and Alternative ApproachĪUTHORS: D. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. ![]() Molecular Cloning: A Laboratory Manual (2nd ed.).
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